Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds
Identifieur interne : 000149 ( Main/Exploration ); précédent : 000148; suivant : 000150Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds
Auteurs : Karine De Guillen [France] ; Cécile Lorrain [France] ; Pascale Tsan [France] ; Philippe Barthe [France] ; Benjamin Petre [France] ; Natalya Saveleva [France] ; Nicolas Rouhier [France] ; Sébastien Duplessis [France] ; André Padilla [France] ; Arnaud Hecker [France]Source :
- Scientific Reports [ 2045-2322 ] ; 2019.
Abstract
Rust fungi are plant pathogens that secrete an arsenal of effector proteins interfering with plant functions and promoting parasitic infection. Effectors are often species-specific, evolve rapidly, and display low sequence similarities with known proteins. How rust fungal effectors function in host cells remains elusive, and biochemical and structural approaches have been scarcely used to tackle this question. In this study, we produced recombinant proteins of eleven candidate effectors of the leaf rust fungus
Url:
DOI: 10.1038/s41598-019-53816-9
PubMed: 31792250
PubMed Central: 6889267
Affiliations:
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486X</institution-id><institution-id institution-id-type="GRID">grid.503276.5</institution-id><institution>Université de Lorraine, INRA, IAM,</institution></institution-wrap>F-54000 Nancy, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>F-54000 Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1763 486X</institution-id><institution-id institution-id-type="GRID">grid.503276.5</institution-id><institution>Université de Lorraine, INRA, IAM,</institution></institution-wrap>F-54000 Nancy, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>F-54000 Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1763 486X</institution-id><institution-id institution-id-type="GRID">grid.503276.5</institution-id><institution>Université de Lorraine, INRA, IAM,</institution></institution-wrap>F-54000 Nancy, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>F-54000 Nancy</wicri:regionArea></affiliation></author><author><name sortKey="Padilla, Andre" sort="Padilla, Andre" uniqKey="Padilla A" first="André" last="Padilla">André Padilla</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0639 1954</institution-id><institution-id institution-id-type="GRID">grid.462825.f</institution-id><institution>Centre de Biochimie Structurale (CBS), INSERM U1054, CNRS UMR 5048, Univ Montpellier,</institution></institution-wrap>F-34090 Montpellier, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>F-34090 Montpellier</wicri:regionArea></affiliation></author><author><name sortKey="Hecker, Arnaud" sort="Hecker, Arnaud" uniqKey="Hecker A" first="Arnaud" last="Hecker">Arnaud Hecker</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1763 486X</institution-id><institution-id institution-id-type="GRID">grid.503276.5</institution-id><institution>Université de Lorraine, INRA, IAM,</institution></institution-wrap>F-54000 Nancy, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>F-54000 Nancy</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Rust fungi are plant pathogens that secrete an arsenal of effector proteins interfering with plant functions and promoting parasitic infection. Effectors are often species-specific, evolve rapidly, and display low sequence similarities with known proteins. How rust fungal effectors function in host cells remains elusive, and biochemical and structural approaches have been scarcely used to tackle this question. In this study, we produced recombinant proteins of eleven candidate effectors of the leaf rust fungus <italic>Melampsora larici-populina</italic> in <italic>Escherichia coli</italic>. We successfully purified and solved the three-dimensional structure of two proteins, MLP124266 and MLP124017, using NMR spectroscopy. Although both MLP124266 and MLP124017 show no sequence similarity with known proteins, they exhibit structural similarities to knottins, which are disulfide-rich small proteins characterized by intricate disulfide bridges, and to nuclear transport factor 2-like proteins, which are molecular containers involved in a wide range of functions, respectively. Interestingly, such structural folds have not been reported so far in pathogen effectors, indicating that MLP124266 and MLP124017 may bear novel functions related to pathogenicity. Our findings show that sequence-unrelated effectors can adopt folds similar to known proteins, and encourage the use of biochemical and structural approaches to functionally characterize effector candidates.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Win, J" uniqKey="Win J">J Win</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petre, B" uniqKey="Petre B">B Petre</name></author><author><name sortKey="Kamoun, S" uniqKey="Kamoun S">S Kamoun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jones, Jdg" uniqKey="Jones J">JDG Jones</name></author><author><name sortKey="Dangl, Jl" uniqKey="Dangl J">JL Dangl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Persoons, A" uniqKey="Persoons A">A Persoons</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="De Guillen, K" uniqKey="De Guillen K">K de Guillen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Win, Joe" uniqKey="Win J">Joe Win</name></author><author><name sortKey="Krasileva, Ksenia V" uniqKey="Krasileva K">Ksenia V. 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Nederveen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laskowski, Ra" uniqKey="Laskowski R">RA Laskowski</name></author><author><name sortKey="Macarthur, Mw" uniqKey="Macarthur M">MW MacArthur</name></author><author><name sortKey="Moss, Ds" uniqKey="Moss D">DS Moss</name></author><author><name sortKey="Thornton, Jm" uniqKey="Thornton J">JM Thornton</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="De Guillen, Karine" sort="De Guillen, Karine" uniqKey="De Guillen K" first="Karine" last="De Guillen">Karine De Guillen</name></noRegion><name sortKey="Barthe, Philippe" sort="Barthe, Philippe" uniqKey="Barthe P" first="Philippe" last="Barthe">Philippe Barthe</name><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><name sortKey="Hecker, Arnaud" sort="Hecker, Arnaud" uniqKey="Hecker A" first="Arnaud" last="Hecker">Arnaud Hecker</name><name sortKey="Lorrain, Cecile" sort="Lorrain, Cecile" uniqKey="Lorrain C" first="Cécile" last="Lorrain">Cécile Lorrain</name><name sortKey="Padilla, Andre" sort="Padilla, Andre" uniqKey="Padilla A" first="André" last="Padilla">André Padilla</name><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name><name sortKey="Saveleva, Natalya" sort="Saveleva, Natalya" uniqKey="Saveleva N" first="Natalya" last="Saveleva">Natalya Saveleva</name><name sortKey="Tsan, Pascale" sort="Tsan, Pascale" uniqKey="Tsan P" first="Pascale" last="Tsan">Pascale Tsan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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